Tyrosine Hydroxylase Antikörper (pSer40)

Produktnummer ABIN5688900

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Tyrosine Hydroxylase in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Schwein und Affe.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (pSer40) (ABIN5688900)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Human, Maus, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Bindungsspezifität: pSer40

Spezifität: Tyrosine Hydroxylase antibody is specific for the 60k rat TH phosphorylated at Ser40 in lysates of PC-12 Cells stimulated by Okadaic Acid (1 μM for 60 minutes). Some higher molecular weight bands may be detected depending upon the brain region being studied, protein loads, and the detection methods used. See cited reference.

Aufreinigung: Affinity Purified

Immunogen: TH (Ser40) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase (TH).

Anwendungsinformationen

Applikationshinweise: Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Human, porcine, quail, mouse, and non-human primate have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase

Hintergrund: Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser40 in rat TH. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.

Molekulargewicht: 60 kDa

Gen-ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour