MARCKS Antikörper (pSer152, pSer156)

Produktnummer ABIN5688889

Der Kaninchen Polyklonal Anti-MARCKS-Antikörper wurde für WB validiert. Er ist geeignet, MARCKS in Proben von Human, Maus, Ratte und Xenopus laevis zu detektieren.

Kurzübersicht für MARCKS Antikörper (pSer152, pSer156) (ABIN5688889)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MARCKS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MARCKS Antikörper

Bindungsspezifität: pSer152, pSer156

Spezifität: MARCKS antibody is specific for MARCKS phosphorylated at Ser152 and Ser156.

Aufreinigung: Affinity Purified

Immunogen: MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.

Anwendungsinformationen

Applikationshinweise: Immunolabeling of the 87  kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Details zu MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Andere Bezeichnung: MARCKS

Hintergrund: Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.

Molekulargewicht: 87 kDa

Gen-ID: 25603

UniProt: P30009