Ficin Antikörper (Biotin)

Produktnummer ABIN568456

Produktdetails anti-Ficin Antikörper (Biotin)

Target: Ficin

Reaktivität: Fig

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ficin Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)

Produktmerkmale: Molar Ratio: Biotin/IgG ~ 6.0

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water

Konzentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu Ficin

Target: Ficin

Abstract: Ficin Produkte

Hintergrund: Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)