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psi-D Subunit of Photosystem I Antikörper

Reaktivität: Chlamydomonas, Oryza sativa, Spinach, Mais (Zea mays) WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5684022
  • Target
    psi-D Subunit of Photosystem I
    Reaktivität
    Chlamydomonas, Oryza sativa, Spinach, Mais (Zea mays)
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Kreuzreaktivität (Details)
    No cross-reactivity with: Synechococcus elongatus
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 17.9 / 20 (for Arabidopsis thaliana)
    PsaD has frequently been used as a marker for intact PSI reaction centers.
    Aufreinigung
    serum
    Immunogen
    KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana (At1g03130 and At1g02770) as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).
  • Applikationshinweise
    Recommended Dilution: 1 : 1000 with SuperSignal West Pico detection (WB).
    Kommentare

    this antibody is a replacement for former product, anti-PsaD AS04 046

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 µL of sterile water
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Heinnickel, Alric, Wittkopp, Yang, Catalanotti, Dent, Niyogi, Wollman, Grossman: "Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7024-36, (2013) (PubMed).

    Yadavalli, Neelam, Rao, Reddy, Subramanyam: "Differential degradation of photosystem I subunits under iron deficiency in rice." in: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).

    Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

  • Target
    psi-D Subunit of Photosystem I
    Andere Bezeichnung
    PSI-D subunit of photosystem I (PsaD)
    Hintergrund
    AGI Code: At1g03130
    PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.
    Molekulargewicht
    expected: 17.9 kDa, apparent: 20 (for Arabidopsis thaliana)
    UniProt
    Q9S7H1, Q9SA56
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