psi-D Subunit of Photosystem I Antikörper

Produktnummer ABIN5684022

Zitiert in 3+ Publikationen. Der Kaninchen Polyklonal anti-psi-D Subunit of Photosystem I Antikörper (ABIN5684022) detektiert spezifisch psi-D Subunit of Photosystem I in CN-PAGE, IP und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana, Gerste, Chlamydomonas reinhardtii, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomytrium Patens, Picea glauca, Pinus strobus, Spinach, Synechocystis, Triticosecale, Triticum aestivum und Mais (Zea mays).

Kurzübersicht für psi-D Subunit of Photosystem I Antikörper (ABIN5684022)

Target: psi-D Subunit of Photosystem I

Reaktivität: Arabidopsis thaliana, Gerste, Chlamydomonas reinhardtii, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomytrium Patens, Picea glauca, Pinus strobus, Spinach, Synechocystis, Triticosecale, Triticum aestivum, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Clear Native PAGE (CN-PAGE), Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-PsaD | PSI-D subunit of photosystem I

Kreuzreaktivität (Details): Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi (red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Oryza sativa, Physcomitrium patens, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays
Not reactive in: Synechococcus elongatus sp. PCC 7942

Homologie: Alge,Dicots,Catalpa bungei,Cucumis melo,Conifers,Cyanidioschyzon merolae,Bigelowiella natans,Nannochloropsis sp.,Phaeodactylum tricornutum,Phyla dulcis,Zosteria marina

Aufreinigung: Serum

Immunogen: KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana PSI-D1 UniProt:Q9S7H1

Anwendungsinformationen

Applikationshinweise: 1: 10 000 (CN-PAGE), 1 : 1000 - 1: 5 000 (WB)

Kommentare:

this antibody is a replacement for former product, anti-PsaD AS04 046

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Heinnickel, Alric, Wittkopp, Yang, Catalanotti, Dent, Niyogi, Wollman, Grossman: "Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7024-36, (2013) (PubMed).

Yadavalli, Neelam, Rao, Reddy, Subramanyam: "Differential degradation of photosystem I subunits under iron deficiency in rice." in: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).

Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

Antigendetails

Target: psi-D Subunit of Photosystem I

Andere Bezeichnung: PSI-D subunit of photosystem I (PsaD)

Hintergrund:

Background: PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.

Additional information: PsaD has frequently been used as a marker for intact PSI reaction centers.This product can be sold containing proclin if requested.

Molekulargewicht: 17.9 | 20 (for Arabidopsis thaliana)

Gen-ID: 838400

UniProt: Q9SA56, Q9S7H1