Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Glucose-6-Phosphate Isomerase (PGI1) in DB, EIA, ID, IF, IP, RIA und WB. Es zeigt Reaktivität gegenüber Proben von Saccharomyces cerevisiae. Es wurde in 4+ Publikationen zitiert.
Kurzübersicht für Glucose-6-Phosphate Isomerase (PGI1) Antikörper (ABIN568369)
Target
Glucose-6-Phosphate Isomerase (PGI1)
Reaktivität
Saccharomyces cerevisiae
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Unkonjugiert
Applikation
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
Spezifität
IgG fraction of polyclonal rabbit antiserum to Phosphoglucose Isomerase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Phosphoglucose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore by adding 1.0 mL of sterile distilled water
Konzentration
10,0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
Cáceres-Gorriti, Carmona, Barrès, Rahimi, Létourneau, Tonin, Provencher, Mes-Masson: "RAN nucleo-cytoplasmic transport and mitotic spindle assembly partners XPO7 and TPX2 are new prognostic biomarkers in serous epithelial ovarian cancer." in: PLoS ONE, Vol. 9, Issue 3, pp. e91000, (2016) (PubMed).
Miura, Ueda: "Evaluation of unconventional protein secretion in Saccharomyces cerevisiae." in: Methods in molecular biology (Clifton, N.J.), Vol. 1270, pp. 51-70, (2015) (PubMed).
Miettinen, Björklund: "Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions." in: Nucleic acids research, Vol. 43, Issue 2, pp. 1019-34, (2015) (PubMed).
Ryu, Kwak, Lee, Kang, Oh, Lee, Kim, Ma, Kim: "Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells." in: Toxicology and applied pharmacology, Vol. 255, Issue 1, pp. 94-102, (2011) (PubMed).