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Alkaline Phosphatase Antikörper

Dieses Anti-Alkaline Phosphatase-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Alkaline Phosphatase in WB, IF, IP, EIA, ID, RIA und DB. Geeignet für E. coli.
Produktnummer ABIN568362

Kurzübersicht für Alkaline Phosphatase Antikörper (ABIN568362)

Target

Alle Alkaline Phosphatase (ALP) Antikörper anzeigen
Alkaline Phosphatase (ALP)

Reaktivität

  • 76
  • 19
  • 14
  • 12
  • 9
  • 1
  • 1
E. coli

Wirt

  • 64
  • 30
  • 2
  • 1
  • 1
Kaninchen

Klonalität

  • 50
  • 48
Polyklonal

Konjugat

  • 48
  • 13
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Dieser Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation

  • 57
  • 36
  • 21
  • 16
  • 12
  • 12
  • 10
  • 9
  • 7
  • 6
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
  • Spezifität

    Alkaline Phosphatase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Aufreinigung

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Alkaline Phosphatase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotyp

    IgG
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Restore by adding 1.0 mL of sterile distilled water

    Konzentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Konservierungsmittel

    Without preservative

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Alkaline Phosphatase (ALP)

    Andere Bezeichnung

    Alkaline Phosphatase

    Hintergrund

    Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: JW0374, b0383, phoA

    Gen-ID

    945041

    NCBI Accession

    AP_001034

    UniProt

    P00634
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