Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Nucleoside Monophosphate Kinase in WB, IF, DB, EIA, ID, IP und RIA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).
Produktnummer ABIN568336
Kurzübersicht für Nucleoside Monophosphate Kinase Antikörper (ABIN568336)
Target
Nucleoside Monophosphate Kinase
Reaktivität
Rind (Kuh)
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Dieser Nucleoside Monophosphate Kinase Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Nucleoside Monophosphate Kinase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore by adding 1 mL of sterile distilled water
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Target
Nucleoside Monophosphate Kinase
Hintergrund
Nucleoside monophosphate (NMP) kinases are ubiquitous enzymes involved in the biosynthesis of nucleotides. Each enzyme catalyses the synthesis of a nucleoside diphosphate that is, in turn, converted to a nucleoside triphosphate by a non-specific nucleoside diphosphate kinase. In prokaryotes, there are five NMP kinases, one for the phosphorylation of each NMP, whereas in eukaryotic organisms, the phosphorylation of both uridine monophosphate (UMP) and cytidine monophosphate (CMP) is carried out by a bifunctional UMP/CMP kinase (Liljelund and Lacroute, 1986).Synonyms: NMPK, Nucleoside monophosphokinase