MDH1 Antikörper (Biotin)

Produktnummer ABIN568317

Dieses Anti-MDH1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MDH1 in WB, IP, IF, EIA, ID, DB und RIA. Geeignet für Schwein.

Kurzübersicht für MDH1 Antikörper (Biotin) (ABIN568317)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDH1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)

Produktdetails anti-MDH1 Antikörper (Biotin)

Spezifität: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Molar Ratio: Biotin/IgG ~4.5

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Malic dehydrogenase is isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1.0 mL sterile distilled water

Konzentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Andere Bezeichnung: MDHA

Hintergrund: Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic

Gen-ID: 396894

NCBI Accession: NP_999039

UniProt: P11708