Luciferase Antikörper (Biotin)

Produktnummer ABIN568305

Dieses Anti-Luciferase-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Luciferase in WB, IF, IP, DB, EIA, RIA und ID. Geeignet für Photinus pyralis.

Kurzübersicht für Luciferase Antikörper (Biotin) (ABIN568305)

Target: Luciferase

Reaktivität: Photinus pyralis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Luciferase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Dot Blot (DB), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID)

Produktdetails anti-Luciferase Antikörper (Biotin)

Produktmerkmale: Molar Ratio: Biotin/IgG: ~1.7

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Luciferase isolated and purified from Photinus pyralis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water.

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu Luciferase

Target: Luciferase

Hintergrund: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase

Gen-ID: 7054

UniProt: P08659