Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA), Dot Blot (DB)
Spezifität
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
D-Lactate dehydrogenase, isolated and purified from Lactobacillus leichmannii. Freund’s complete adjuvant is used in the first step of the immunization procedure.