Der Kaninchen Polyklonal Anti-ACSS2 Antikörper wurde für WB, IF, IP, EIA, DB, ID und RIA validiert. Er ist geeignet, ACSS2 in Proben von Saccharomyces cerevisiae zu detektieren.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Aufreinigung
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
Purified S-Acetyl Coenzyme A Synthetase from baker's Yeast
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore by adding 1.0 mL of sterile distilled water.
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Target
ACSS2
(Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))
Andere Bezeichnung
ACSS2
Hintergrund
Acetyl Coenzyme A Synthetase is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. It acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: ACAS2, ACSA, Acetate-CoA ligase, Acetyl-CoA synthetase, Acetyl-coenzyme A synthetase cytoplasmic, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme