Fibronectin Antikörper

Produktnummer ABIN5680165

Der Kaninchen Polyklonal Anti-Fibronectin-Antikörper wurde für WB, IF, ELISA, IHC (p) und RIA validiert. Er ist geeignet, Fibronectin in Proben von Ratte zu detektieren.

Kurzübersicht für Fibronectin Antikörper (ABIN5680165)

Target: Fibronectin

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Produktdetails anti-Fibronectin Antikörper

Spezifität: Rat fibronectin 100 % , rat collagen type I, II, III, IV and V <0.1 %, rat elastin <0.1 % (RIA at 1:5000 dilution)

Kreuzreaktivität (Details): Rat

Produktmerkmale: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Rat fibronectin 100 % , rat collagen type I, II, III, IV and V <0.1 %, rat elastin <0.1 % (RIA at 1:5000 dilution).

Aufreinigung: Affinity purified

Immunogen: Purified fibronectin from rat plasma

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Rat skin,IHC(P), IFA, ELISA, RIA, IB (WB),IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:200 (OD ? 500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Konzentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu Fibronectin

Target: Fibronectin