Fibronectin Antikörper

Produktnummer ABIN5680162

Der Kaninchen Polyklonal Anti-Fibronectin-Antikörper wurde für IF, ELISA, IHC (p) und RIA validiert. Er ist geeignet, Fibronectin in Proben von Huhn zu detektieren.

Kurzübersicht für Fibronectin Antikörper (ABIN5680162)

Target: Fibronectin

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Produktdetails anti-Fibronectin Antikörper

Spezifität: Chicken fibronectin 100 % , chicken collagen type I, III and V <0.1 % (RIA at 1:10000 dilution)

Kreuzreaktivität (Details): Chicken

Produktmerkmale: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Chicken fibronectin 100 % , chicken collagen type I, III and V <0.1 % (RIA at 1:10000 dilution).

Aufreinigung: Affinity purified

Immunogen: Purified fibronectin from chicken plasma

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Chicken skin,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Konzentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu Fibronectin

Target: Fibronectin