Rekombinanter Flavivirus Group Antigen Antikörper

Produktnummer ABIN5668106

Produktdetails

Target: Flavivirus Group Antigen

Antikörpertyp: Recombinant Antibody

Reaktivität: Dengue Virus, Flavivirus, Virus, West Nile Virus (WNV), Gelbfieber-Virus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Spezifität: Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.

Produktmerkmale:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Aufreinigung: Purified antibody.

Immunogen: Dengue Virus type 2 antigens.

Klon: D1-4G2-4-15 (4G2)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Referenzen

Conzelmann, Zou, Groß, Harms, Röcker, Riedel, Münch, Müller: "Storage-Dependent Generation of Potent Anti-ZIKV Activity in Human Breast Milk." in: Viruses, Vol. 11, Issue 7, (2020) (PubMed).

Müller, Harms, Krüger, Groß, Joas, Hayn, Dietz, Lippold, von Einem, Schubert, Michel, Mayer, Cortese, Jang, Sandi-Monroy, Deniz, Ebner, Vapalahti, Otto, Bartenschlager, Herbeuval, Schmidt-Chanasit et al.: "Semen inhibits Zika virus infection of cells and tissues from the anogenital region. ..." in: Nature communications, Vol. 9, Issue 1, pp. 2207, (2018) (PubMed).

Wang, Bardelli, Espinosa, Pedotti, Ng, Bianchi, Simonelli, Lim, Foglierini, Zatta, Jaconi, Beltramello, Cameroni, Fibriansah, Shi, Barca, Pagani, Rubio, Broccoli, Vicenzi, Graham, Pullan, Dowall et al.: "A Human Bi-specific Antibody against Zika Virus with High Therapeutic Potential. ..." in: Cell, Vol. 171, Issue 1, pp. 229-241.e15, (2017) (PubMed).

Antigendetails

Target: Flavivirus Group Antigen

Substanzklasse: Viral Protein

Hintergrund: Protein E, Envelope Protein