Rekombinanter Polysialic Acid Antikörper

Produktnummer ABIN5668052

Zitiert in 1 Publikation. Der Maus Monoklonal anti-Polysialic Acid Antikörper (Klon 735) (ABIN5668052) detektiert spezifisch Polysialic Acid in FACS, IHC, IP und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Rekombinanter Polysialic Acid Antikörper (ABIN5668052)

Target: Polysialic Acid

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Klon: 735

Produktdetails

Verwendungszweck: Anti-Polysialic acid [735], Mouse IgG2a, kappa

Spezifität: Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.

Kreuzreaktivität: Maus, Ratte

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: Homopolymers of alpha2,8-linked sialic acid of Escherichia coli K1.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989, PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000, PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Referenzen

Tytgat, Lin, Levasseur, Tomek, Rutschmann, Mock, Liebscher, Terasaka, Azuma, Wetter, Bachmann, Hilvert, Aebi, Keys: "Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies." in: Nature communications, Vol. 10, Issue 1, pp. 5403, (2020) (PubMed).

Antigendetails

Target: Polysialic Acid

Hintergrund: PSA, N-CAM, neural cell adhesion molecule, polySA