KDEL Antikörper (N-Term)

Produktnummer ABIN5650489

Dieses Anti-KDEL-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KDEL in WB und IHC. Geeignet für Maus, Ratte und Human.

Kurzübersicht für KDEL Antikörper (N-Term) (ABIN5650489)

Target: KDEL (Lys Asp Glu Leu)

Reaktivität: Maus, Ratte, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KDEL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 2C1

Produktdetails anti-KDEL Antikörper

Bindungsspezifität: N-Term

Spezifität: Detects KDEL tagged proteins.

Aufreinigung: Protein G Purified

Immunogen: KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5650489 was sufficient for detection of KDEL in 10 μg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

Lagerung: -20 °C

Details zu KDEL

Target: KDEL (Lys Asp Glu Leu)

Andere Bezeichnung: KDEL

Hintergrund: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.