Mast Cell Tryptase Antikörper

Produktnummer ABIN5647716

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Mast Cell Tryptase in IHC (p), WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Mast Cell Tryptase Antikörper (ABIN5647716)

Target: Mast Cell Tryptase

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Mast Cell Tryptase Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Produktdetails anti-Mast Cell Tryptase Antikörper

Aufreinigung: Antigen affinity purified

Immunogen: Amino acids H65-P275 from the human protein were used as the immunogen for the Mast Cell Tryptase antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,ELISA: 0.1-0.5 μg/mL (human protein tested), request BSA-free format for coating

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: Prior to reconstitution, store at 4°C. After reconstitution, the Mast Cell Tryptase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Mast Cell Tryptase

Target: Mast Cell Tryptase

Hintergrund: Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

UniProt: Q15661