Prohibitin 1 / PHB1 (AA 167-261) Antikörper

Produktnummer ABIN5646917

Dieser Anti- Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von in IHC (p), ELISA und WB. Geeignet für Human.

Kurzübersicht für Prohibitin 1 / PHB1 (AA 167-261) Antikörper (ABIN5646917)

Target: Prohibitin 1 / PHB1

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)

Klon: PHB-1882

Produktdetails

Bindungsspezifität: AA 167-261

Produktmerkmale: This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Aufreinigung: Purified

Reinheit: Protein G affinity chromatography

Immunogen: A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PHB antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL,ELISA: 2-4 μg for coating using the BSA-free format),Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the PHB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Prohibitin 1 / PHB1

Hintergrund: This antibody recognizes a protein of 30  kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.