Dieser Maus Monoklonal Antikörper erkennt spezifisch CKB in FACS, IF und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Hund, Zebrafisch (Danio rerio), Rind (Kuh), Huhn, Frosch und Rhesusaffen.
Optimal dilution of the Creatine kinase B type antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen Only): 0.5-1 μg/mL for 30 min at RT
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.2 mg/mL
Buffer
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store the Creatine kinase B type antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Target
CKB
(Creatine Kinase, Brain (CKB))
Andere Bezeichnung
Creatine kinase B type / CKB
Hintergrund
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins