Histone H4 Antikörper (N-Term)

Produktnummer ABIN5596994

Hersteller Produkt- Nr.: 100-401-br9

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Histone H4 in WB, ELISA und DB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Histone H4 Antikörper (N-Term) (ABIN5596994)

Target: Histone H4

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB)

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 100-401-br9

Hersteller: Rockland

Verwendungszweck: Histone H4 Antibody

Kreuzreaktivität (Details): Cross reactivity with other species was not tested.

Produktmerkmale: Synonyms: rabbit anti-Histone H4 antibody, Histone H4, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4J, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Aufreinigung: Anti-Histone H4 pan Antibody antiserum is directed against Histone H4.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Histone H4 pan Antibody is tested for Western Blot and Dot Blots. This antibody is suitable for Chromatin Immunoprecipitation and ELISA. Expect a single band approximately 11.3 kDa in size corresponding to H4 by western blot in the appropriate tissue or cell lysate. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:2000

ELISA Dilution: 1:20000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone H4

Target: Histone H4

Hintergrund: Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Gen-ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805