Uricase (ABC3735) Antikörper (HRP)

Produktnummer ABIN5596984

Hersteller Produkt- Nr.: 200-103-092-0100

Dieses Anti--Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von in ELISA, IP und WB. Geeignet für Bacillus.

Kurzübersicht für Uricase (ABC3735) Antikörper (HRP) (ABIN5596984)

Target: Uricase (ABC3735)

Reaktivität: Bacillus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Hersteller Produkt- Nr.: 200-103-092-0100

Hersteller: Rockland

Verwendungszweck: Uricase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].

Produktmerkmale: Synonyms: goat anti-uricase antibody, HRP conjugated goat anti-uricase antibody, Urate oxidase antibody

Aufreinigung: Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: Uricase (ABC3735)

Andere Bezeichnung: ABC3735

Substanzklasse: Dinucleotide

Hintergrund: Background: Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localized in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form, and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.

Gen-ID: 3202700

NCBI Accession: YP_177228

UniProt: Q5WBJ3