Protein G Antikörper (HRP)

Produktnummer ABIN5596974

Hersteller Produkt- Nr.: 200-403-138-0100

Der Kaninchen Polyklonal Anti-Protein G-Antikörper wurde für ELISA und DB validiert. Er ist geeignet, Protein G in Proben von Streptococcus zu detektieren.

Kurzübersicht für Protein G Antikörper (HRP) (ABIN5596974)

Target: Protein G

Reaktivität: Streptococcus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Protein G Antikörper ist konjugiert mit HRP

Applikation: ELISA, Dot Blot (DB)

Produktdetails anti-Protein G Antikörper (HRP)

Hersteller Produkt- Nr.: 200-403-138-0100

Hersteller: Rockland

Verwendungszweck: Protein G Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].

Produktmerkmale: Synonyms: rabbit anti-Protein G Antibody HRP Conjugation, Peroxidase conjugated rabbit anti-Protein G Antibody, Protein G HRP

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Protein G [Streptococcus species]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Protein G Peroxidase Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Protein G [Streptococcus species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:20,000 - 1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Protein G

Target: Protein G

Hintergrund: Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein G Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.