R-Phycoerythrin Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN5596963

Produktdetails anti-R-Phycoerythrin Antikörper (Alkaline Phosphatase (AP))

Target: R-Phycoerythrin (Phycoerythrin, R-)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser R-Phycoerythrin Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität (Details): This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Referenzen für anti-R-Phycoerythrin Antikörper (Alkaline Phosphatase (AP)) (ABIN5596963)

Beute, Lukkes, Koekoek, Nastiti, Ganesh, de Bruijn, Hockman, van Nimwegen, Braunstahl, Boon, Lambrecht, Manganiello, Hendriks, KleinJan: "A pathophysiological role of PDE3 in allergic airway inflammation." in: JCI insight, Vol. 3, Issue 2, (2018) (PubMed).

Details zu R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Andere Bezeichnung: R-Phycoerythrin (R-Phycoerythrin Produkte)

Substanzklasse: Chemical

Hintergrund:

Synonyms: ALP, Alk Phos