Luciferase Antikörper (HRP)

Produktnummer ABIN5596940

Hersteller Produkt- Nr.: 200-103-150-0100

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Luciferase in WB und ELISA. Er zeigt eine Reaktivität gegenüber Photinus pyralis und wurde in 1 Publikation erwähnt.

Kurzübersicht für Luciferase Antikörper (HRP) (ABIN5596940)

Target: Luciferase

Reaktivität: Photinus pyralis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Luciferase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Luciferase Antikörper (HRP)

Hersteller Produkt- Nr.: 200-103-150-0100

Hersteller: Rockland

Verwendungszweck: Luciferase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].

Produktmerkmale: Synonyms: goat anti-Luciferase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Luciferase Antibody

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Luciferase [Photinus pyralis (Firefly)]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Anti-Luciferase Peroxidase has been tested by ELISA and western blot. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract. Anti-Luciferase has been assayed against 1.0 μg of Luciferase [Photinus pyralis (Firefly)] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:5,000 - 1:10,000

ELISA Dilution: 1:50,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-Luciferase Antikörper (HRP) (ABIN5596940)

Windahl, Lagerquist, Andersson, Jochems, Kallkopf, Håkansson, Inzunza, Gustafsson, van der Saag, Carlsten, Pettersson, Ohlsson: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).

Details zu Luciferase

Target: Luciferase

Hintergrund: Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.