Laminin Antikörper (Biotin)

Produktnummer ABIN5596937

Hersteller Produkt- Nr.: 600-406-116

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Laminin in WB, IHC, ELISA und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Laminin Antikörper (Biotin) (ABIN5596937)

Target: Laminin (LN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Laminin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Laminin Antikörper (Biotin)

Hersteller Produkt- Nr.: 600-406-116

Hersteller: Rockland

Verwendungszweck: Laminin Antibody Biotin Conjugated

Kreuzreaktivität (Details): Typically less than 1 % cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.

Produktmerkmale: Synonyms: rabbit anti-Laminin antibody biotin conjugation, biotin conjugated rabbit anti-Laminin antibody, LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody

Aufreinigung: Anti-Laminin has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen:

Immunogen: Non-reduced 400 kDa human laminin.  Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Laminin biotin conjugated antibody is suitable for western blotting, IHC, IP and for ELISA and for use with streptavidin reporter reagents. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:20,000 - 1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  

Haltbarkeit: 12 months

Details zu Laminin

Target: Laminin (LN)

Andere Bezeichnung: Laminin

Hintergrund: Background: Anti-Laminin (biotin conjugated) detects laminin. The biotin conjugation makes anti-laminin antibody suitable for use with streptavidin detection systems. Laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. The laminins are a family of glycoproteins that are an integral part of the structural scaffolding in almost every tissue of an organism. They are secreted and incorporated into cell-associated extracellular matrices. Defective laminins can cause muscles to form improperly, leading to a form of muscular dystrophy, lethal skin blistering disease and defects of the kidney filter. Anti-Laminin Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.

Gen-ID: 3912

UniProt: P07942