FOLR1 Antikörper (HRP)

Produktnummer ABIN5596925

Hersteller Produkt- Nr.: 200-103-148-0100

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch FOLR1 in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für FOLR1 Antikörper (HRP) (ABIN5596925)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reaktivität: Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FOLR1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-FOLR1 Antikörper (HRP)

Hersteller Produkt- Nr.: 200-103-148-0100

Hersteller: Rockland

Verwendungszweck: Folate Binding Protein Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].

Produktmerkmale: Synonyms: goat anti-Folate Binding Protein Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP

Aufreinigung: Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Folate Binding Protein [Bovine Milk]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Folate Binding Protein has been tested in western blot and is suitable to be assayed against 1.0 μg of Folate Binding Protein [Bovine Milk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Andere Bezeichnung: FOLR1

Hintergrund: Background: Folate-binding protein (FBP) are proteins that bind folate, typically the folate receptors and reduced folate carrier. In cancer research it can be a marker for ovarian cancer. It can be measured to help in tumor classification to aid treatment. FBP is over-expressed (20-80 fold) in more than 90 % of ovarian and endometrial cancers, as well as 20-50 % of breast, lung, colorectal, and renal cell carcinomas. FBP has very limited tissue distribution and expression in non-malignant tissue, making it a good immunotherapy target.

Gen-ID: 539750

NCBI Accession: NP_001193456

UniProt: P02702

Pathways: Dicarboxylic Acid Transport