Trypsin Inhibitor Antikörper (HRP)

Produktnummer ABIN5596918

Hersteller Produkt- Nr.: 200-4387-0100

Der HRP-konjugierte Kaninchen Polyklonal anti-Trypsin Inhibitor Antikörper (ABIN5596918) detektiert spezifisch Trypsin Inhibitor in WB, IP und DB. Dieser Antikörper reagiert spezifisch mit Proben aus Lima bean.

Kurzübersicht für Trypsin Inhibitor Antikörper (HRP) (ABIN5596918)

Target: Trypsin Inhibitor

Reaktivität: Lima bean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsin Inhibitor Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Produktdetails anti-Trypsin Inhibitor Antikörper (HRP)

Hersteller Produkt- Nr.: 200-4387-0100

Hersteller: Rockland

Verwendungszweck: Trypsin Inhibitor Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Produktmerkmale: Synonyms: rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Aufreinigung: Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Trypsin Inhibitor [Lima Bean]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Trypsin Inhibitor Peroxidase Conjugated Antibody has been tested by Dot blot and is suitable to be assayed against 1.0 μg of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

Immunoprecipitation Dilution: 1:100

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Trypsin Inhibitor

Target: Trypsin Inhibitor

Hintergrund: Background: Trypsin Inhibitors are chemicals that reduce the availability of biologically active trypsin, an enzyme essential to nutrition of many animals, including humans. Tumor Associated Trypsin Inhibitor (TATI) has been used as a marker for various carcinomas and useful in cancer diagnosis.

Gen-ID: 547831

UniProt: P01070