CPB1 Antikörper (HRP)

Produktnummer ABIN5596913

Hersteller Produkt- Nr.: 200-403-049-0100

Produktdetails anti-CPB1 Antikörper (HRP)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPB1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-403-049-0100

Hersteller: Rockland

Verwendungszweck: Carboxypeptidase B Antibody HRP Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Carboxypeptidase B antibody HRP conjugation, peroxidase conjugated rabbit anti-Carboxypeptidase B Antibody, CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

Aufreinigung: Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carboxypeptidase B [Porcine Pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Carboxypeptidase B peroxidase Antibody has been tested in western blot and suitable to be assayed against 1.0 μg of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody.

Western Blot Dilution: 1:250 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Andere Bezeichnung: CPB1 (CPB1 Produkte)

Hintergrund: Background: Anti-Carboxypeptidase B Antibody is a key enzyme in the hydrolysis in basic amino acids in the C-terminal position of polypeptides. First isolated from the porcine pancreas, Carboxypeptidase B is activated by trypsin and exhibits high specificity for lysine and arginine. Carboxypeptidase B is also a key enzyme in insulin product in humans and can be used as a marker for pancreatic diagnosis. Anti-Carboxypeptidase B is ideal for investigators in Cell Biology, Immunology, and Microbiology.

Gen-ID: 397341

NCBI Accession: NP_999334

UniProt: P09955