Glucose-6-Phosphate Dehydrogenase Antikörper (HRP)

Produktnummer ABIN5596888

Hersteller Produkt- Nr.: 200-1353-0100

Dieses Anti-Glucose-6-Phosphate Dehydrogenase-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Glucose-6-Phosphate Dehydrogenase in WB und ELISA. Geeignet für Leuconostoc mesenteroides.

Kurzübersicht für Glucose-6-Phosphate Dehydrogenase Antikörper (HRP) (ABIN5596888)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Leuconostoc mesenteroides

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper (HRP)

Hersteller Produkt- Nr.: 200-1353-0100

Hersteller: Rockland

Verwendungszweck: Glucose-6-Phosphate Dehydrogenase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate-Dehydrogenase [Yeast].

Produktmerkmale: Synonyms: goat anti-Glucose-6-Phosphate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody

Aufreinigung: Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glucose-6-Phosphate-Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Glucose-6-Phosphate Dehydrogenase has been tested in ELISA and western blot. This product is assayed against 1.0 μg of Glucose-6-Phosphate-Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: Glucose-6-Phosphate Dehydrogenase

Hintergrund: Background: Glucose-6-Phosphate Dehydrogenase is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH), which maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Cell growth and proliferation are affected by Glucose-6-Phosphate Dehydrogenase. Glucose-6-Phosphate Dehydrogenase inhibitors are under investigation to treat cancers and other conditions. DHEA is a Glucose-6-Phosphate Dehydrogenase inhibitor.

Gen-ID: 6063509

UniProt: P11411

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones