Acetylated Lysine Antikörper

Produktnummer ABIN5596809

Hersteller Produkt- Nr.: 600-401-939

Der Kaninchen Polyklonal Anti-Acetylated Lysine-Antikörper wurde für WB, ELISA, IHC, IP, FACS und FM validiert. Er ist geeignet, Acetylated Lysine in Proben von Diverse Spezies zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Acetylated Lysine Antikörper (ABIN5596809)

Target: Acetylated Lysine

Reaktivität: Diverse Spezies

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Acetylated Lysine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Produktdetails anti-Acetylated Lysine Antikörper

Hersteller Produkt- Nr.: 600-401-939

Hersteller: Rockland

Verwendungszweck: Lysine Acetylated Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Produktmerkmale: Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Aufreinigung: Anti-Lysine Acetylated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Lysine Acetylated Antibody is suitable for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, flow cytometry, and immunohistochemistry.

Western Blot Dilution: 1:500-1:1,000

Immunoprecipitation Dilution: 10 μg/mg protein sample

ELISA Dilution: 1:1,000-1:2,500

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Acetylated Lysine Antikörper (ABIN5596809)

Zhou, Huang, Zhang, Zhao, Kim, Tu, Zhang, Nowsheen, Zhu, Deng, Chen, Qin, Luo, Liu, Lou, Mutter, Yuan: "The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair." in: Nature communications, Vol. 11, Issue 1, pp. 2639, (2020) (PubMed).

Lin, Yuan, Pei, Liu, Ann, Lou: "KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123935, (2016) (PubMed).

Pérez-Luna, Aguasca, Perearnau, Serratosa, Martínez-Balbas, Jesús Pujol, Bachs: "PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1." in: Nucleic acids research, Vol. 40, Issue 14, pp. 6520-33, (2012) (PubMed).

Details zu Acetylated Lysine

Target: Acetylated Lysine

Andere Bezeichnung: Lysine Acetylated

Substanzklasse: Chemical

Hintergrund: Background: Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.