Ascorbate Oxidase Antikörper (HRP)

Produktnummer ABIN5596757

Hersteller Produkt- Nr.: 200-4348-0100

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ascorbate Oxidase in ELISA, WB und IP. Er zeigt eine Reaktivität gegenüber Pumpkin.

Kurzübersicht für Ascorbate Oxidase Antikörper (HRP) (ABIN5596757)

Target: Ascorbate Oxidase (AO)

Reaktivität: Pumpkin

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ascorbate Oxidase Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-Ascorbate Oxidase Antikörper (HRP)

Hersteller Produkt- Nr.: 200-4348-0100

Hersteller: Rockland

Kreuzreaktivität (Details): Cross reactivity against Ascorbate Oxidase from other sources is unknown.

Aufreinigung: Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].

Immunogen:

Immunogen: Ascorbate Oxidase [cucurbita]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Ascorbate Oxidase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Andere Bezeichnung: Ascorbate Oxidase

Hintergrund:

Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3

Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.

Gene Name: AAO

UniProt: P24792