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LPO Antikörper (HRP)

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch LPO in WB, IP, ELISA und DB. Er zeigt eine Reaktivität gegenüber Rind (Kuh).
Rockland
Produktnummer ABIN5596738
Hersteller Produkt- Nr.: 200-603-146-0100

Kurzübersicht für LPO Antikörper (HRP) (ABIN5596738)

Target

Alle LPO Antikörper anzeigen
LPO (Lactoperoxidase (LPO))

Reaktivität

  • 11
  • 9
  • 8
  • 3
  • 3
  • 2
  • 1
Rind (Kuh)

Wirt

  • 19
  • 5
  • 3
Schaf

Klonalität

  • 22
  • 5
Polyklonal

Konjugat

  • 18
  • 8
  • 1
Dieser LPO Antikörper ist konjugiert mit HRP

Applikation

  • 24
  • 18
  • 16
  • 14
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), ELISA, Dot Blot (DB)
  • Hersteller Produkt- Nr.

    200-603-146-0100

    Hersteller

    Rockland

    Verwendungszweck

    Lactoperoxidase Antibody Peroxidase Conjugated

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].

    Produktmerkmale

    Synonyms: sheep anti-Lactoperoxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

    Aufreinigung

    Lactoperoxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogen

    Immunogen: Lactoperoxidase [Bovine Milk]

    Immunogen Type: Native Protein

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: Anti-Lactoperoxidase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase [Bovine Milk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase Antibody.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    10.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Konservierungsmittel

    Gentamicin sulfate

    Vorsichtsmaßnahmen

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Target

    LPO (Lactoperoxidase (LPO))

    Andere Bezeichnung

    Lactoperoxidase

    Hintergrund

    Background: Lactoperoxidase antibody recognizes the lactoperoxidase protein. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. Lactoperoxidase plays an important role in killing bacteria in milk. Lactoperoxidase conjugated to peroxidase is used to bind to the heavy and light specific target of the lactoperoxidase protein. Lactoperoxidase conjugated to Peroxidase is suitable for researchers in immunology, cancer, and biochemistry.

    Gen-ID

    280844

    UniProt

    P80025
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