Elastase Antikörper (HRP)

Produktnummer ABIN5596709

Hersteller Produkt- Nr.: 200-4368-0100

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Elastase in ELISA, WB und IHC. Er zeigt eine Reaktivität gegenüber Schwein.

Kurzübersicht für Elastase Antikörper (HRP) (ABIN5596709)

Target: Elastase

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Elastase Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Elastase Antikörper (HRP)

Hersteller Produkt- Nr.: 200-4368-0100

Hersteller: Rockland

Verwendungszweck: Elastase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Elastase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

Aufreinigung: Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Elastase [Porcine Pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Elastase Peroxidase conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Elastase [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Elastase

Target: Elastase

Hintergrund: Background: Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. Elastase also has the important immunological role of breaking down Shigella virulence factors. Elastase is responsible for pulmonary emphysema, cyclic hematopoiesis, and blistering in bullous pemphigoid.

Gen-ID: 396766

NCBI Accession: NP_998988

UniProt: P00772