DLD Antikörper (HRP)

Produktnummer ABIN5596706

Produktdetails anti-DLD Antikörper (HRP)

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DLD Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Lipoamide Dehydrogenase from other sources is unknown.

Aufreinigung: Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

Immunogen:

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Lipoamide Dehydrogenase has been assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Andere Bezeichnung: Lipoamide Dehydrogenase (DLD Produkte)

Synonyme: DLDD antikoerper, DLDH antikoerper, E3 antikoerper, GCSL antikoerper, LAD antikoerper, PHE3 antikoerper, AI315664 antikoerper, AI746344 antikoerper, wu:fb24b05 antikoerper, DLD antikoerper, DDBDRAFT_0183800 antikoerper, DDBDRAFT_0216232 antikoerper, DDB_0183800 antikoerper, DDB_0216232 antikoerper, sc:d0402 antikoerper, dihydrolipoamide dehydrogenase antikoerper, dihydrolipoyl dehydrogenase antikoerper, deltaD antikoerper, DLD antikoerper, Dld antikoerper, dldh antikoerper, AT4G16155 antikoerper, CND05840 antikoerper, bfmBC antikoerper, GCSL antikoerper, LACBIDRAFT_182385 antikoerper, UREG_06178 antikoerper, lpd antikoerper, TAGG_RS02070 antikoerper, Arnit_2606 antikoerper, Mesil_1945 antikoerper, Trad_2118 antikoerper, Acear_0640 antikoerper, Fbal_0372 antikoerper, Ilyop_1890 antikoerper, Ftrac_1733 antikoerper, Ocepr_1753 antikoerper, Intca_2017 antikoerper, Deima_0504 antikoerper, dld antikoerper

Hintergrund:

Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial EC=1.8.1.4 Dihydrolipoamide dehydrogenase Lipoamide Dehydrogenase [Porcine Heart]

Background: Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Gene Name: DLD LAD

Gen-ID: 397129

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis