Mutarotase Antikörper (HRP)
-
- Target
- Mutarotase
-
Reaktivität
- Schwein
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- HRP
-
Applikation
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Hersteller Produkt- Nr.
- 200-4362-0100
- Hersteller
- Rockland
- Verwendungszweck
- Mutarotase Antibody Peroxidase Conjugated
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Mutarotase [Porcine Kidney].
- Produktmerkmale
- Synonyms: rabbit anti-Mutarotase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Mutarotase Antibody, Aldose 1 epimerase antibody, BLOCK25 protein antibody, Galactose mutarotase antibody, GALM antibody
- Aufreinigung
- Mutarotase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
-
Immunogen: Mutarotase [Porcine Kidney]
Immunogen Type: Native Protein
- Isotyp
- IgG
-
-
- Applikationshinweise
-
Application Note: Anti-Mutarotase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Mutarotase [Porcine Kidney] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - Konservierungsmittel
- Gentamicin sulfate
- Vorsichtsmaßnahmen
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
-
- Target
- Mutarotase
- Hintergrund
- Background: Mutarotase is found especially in mammalian tissues that catalyzes the interconversion of anomeric forms of some sugars: α- and β- forms of d-glucose, l-arabinose, d-galactose, d-xylose, lactose, and maltose.
- Gen-ID
- 399536
- UniProt
- Q9GKX6
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