Papain Antikörper (HRP)

Produktnummer ABIN5596693

Hersteller Produkt- Nr.: 200-1374-0100

Produktdetails

Target: Papain

Reaktivität: Papaya

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-1374-0100

Hersteller: Rockland

Verwendungszweck: Papain Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].

Produktmerkmale: Synonyms: goat anti-Papain Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

Aufreinigung: Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200- 1:1,000

Application Note: Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500- 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: Papain

Hintergrund: Background: Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

UniProt: P00784