Rekombinanter Rab6-GTP (Gln72Leu-Mutant) Antikörper (Atto 488)

Produktnummer ABIN5564232

Der Human Monoklonal Anti--Antikörper wurde für ICC validiert. Er ist geeignet, in Proben von Drosophila melanogaster, Human und Maus zu detektieren.

Kurzübersicht für Rekombinanter Rab6-GTP (Gln72Leu-Mutant) Antikörper (Atto 488) (ABIN5564232)

Target: Rab6-GTP

Antikörpertyp: Recombinant Antibody

Reaktivität: Drosophila melanogaster, Human, Maus

Wirt: Human

Klonalität: Monoklonal

Konjugat: Atto 488

Applikation: Immunocytochemistry (ICC)

Klon: AA2

Produktdetails

Bindungsspezifität: Gln72Leu-Mutant

Spezifität: Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L.

Kreuzreaktivität: Fruchtfliege (Drosophila melanogaster), Human, Maus

Kreuzreaktivität (Details): Does not detect Rab6•GDP.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu.

Isotyp: IgG2

Anwendungsinformationen

Kommentare:

anti-Rab6-GTP, monoclonal antibody (recombinant) (AA2) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Rab6-GTP, monoclonal antibody (recombinant) (AA2) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: +4°C
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.

Haltbarkeit: 12 months

Antigendetails

Target: Rab6-GTP

Hintergrund: Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).

UniProt: P20340