SNCA Antikörper (PE)

Produktnummer ABIN5564087

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SNCA in WB, ELISA, IF, ICC und DB. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für SNCA Antikörper (PE) (ABIN5564087)

Target: SNCA (Synuclein, alpha (SNCA))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SNCA Antikörper ist konjugiert mit PE

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Klon: 10H7

Produktdetails anti-SNCA Antikörper (PE)

Spezifität: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.,Binds within the 101-130 aa of human ASYN, determined using DB and ELISA with corresponding peptides. Detects ~14 kDa band corr to ASYN. ~40 kDa is a trimer. Does not detect truncated ASYN (aa 1-114)

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Mouse alpha synuclein aggregate

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• DB (1:1000)
• ICC/IF (1:100)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN5564087 was sufficient for detection of Alpha Synuclein in 15 μg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Andere Bezeichnung: Alpha Synuclein

Hintergrund: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Gen-ID: 20617

NCBI Accession: NP_001035916

UniProt: O55042

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity