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TRIM25 Antikörper (AA 211-360)

Name: TRIM25 Antikörper (AA 211-360)
Brand: antibodies-online
SKU: ABIN5542675
Price: 640.59 EUR
Availability: InStock

Dieses Anti-TRIM25-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TRIM25 in WB, ELISA, IHC, ICC und FACS. Geeignet für Human.

Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate. (TRIM25 Antikörper (AA 211-360))

Flow cytometric analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red). (TRIM25 Antikörper (AA 211-360))

FACS

Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). (TRIM25 Antikörper (AA 211-360))

ICC

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining. (TRIM25 Antikörper (AA 211-360))

IHC

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining. (TRIM25 Antikörper (AA 211-360))

IHC

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) (TRIM25 Antikörper (AA 211-360))

ELISA

Western blot analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (TRIM25 Antikörper (AA 211-360))

Western blot analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate. (TRIM25 Antikörper (AA 211-360))

8 Abbildungen

Produktnummer ABIN5542675

Datenblatt (PDF)

Kurzübersicht für TRIM25 Antikörper (AA 211-360) (ABIN5542675)

Target

Alle TRIM25 Antikörper anzeigen

TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reaktivität

Human

Wirt

Maus

Klonalität

Monoklonal

Konjugat

Dieser TRIM25 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon

5B5B12

Bindungsspezifität
15

15

7

6

5

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 211-360

Verwendungszweck

TRIM25 Antibody

Aufreinigung

Purified antibody

Immunogen

Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.

Isotyp

IgG2b
anti-Tripartite Motif Containing 25 (TRIM25) (AA 100-400) antibody
TRIM25 Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 211-310) antibody
TRIM25 Reaktivität: Human WB, ELISA, IF, IHC (p) Wirt: Maus Monoclonal 5C3 unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 81-272) antibody
TRIM25 Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 1-630) antibody
TRIM25 Reaktivität: Human WB, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) antibody
TRIM25 Reaktivität: Human WB, IF, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 1-630) antibody
TRIM25 Reaktivität: Human WB, IF Wirt: Maus Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 32-210) antibody
TRIM25 Reaktivität: Human WB, IHC, IF, IP Wirt: Maus Monoclonal 2-EFP unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (C-Term) antibody
TRIM25 Reaktivität: Human, Maus WB, ELISA, IHC, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 243-491) antibody
TRIM25 Reaktivität: Human WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Tripartite Motif Containing 25 (TRIM25) (AA 531-630) antibody
TRIM25 Reaktivität: Human, Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/250

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Target

TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Andere Bezeichnung

TRIM25

Hintergrund

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Molekulargewicht

71 kDa

UniProt

Q14258