Transferrin Antikörper (N-Term)

Produktnummer ABIN5542240

Dieser Anti-Transferrin Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Transferrin in WB, ICC, IHC (p), IF, EIA, RIA und Func. Geeignet für Human, Schwein und Kaninchen.

Kurzübersicht für Transferrin Antikörper (N-Term) (ABIN5542240)

Target: Transferrin (TF)

Reaktivität: Human, Schwein, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Functional Studies (Func)

Klon: HTF-14

Produktdetails anti-Transferrin Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Kreuzreaktivität (Details): Does not react with Bovine, Sheep, Canine (Dog), Equine (Horse).

Aufreinigung: Precipitation methods and ion exchange chromatography (> 95% pure by SDS-PAGE)

Immunogen: Purified porcine transferrin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot : non-reducing conditions. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive control: placenta. Immunocytochemistry. ELISA. RIA. Functional application: blocks binding of transferrin to the receptor.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 95 % pure by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS), approx. pH 7.4 0.2 μm filter sterilized

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: transferrin

Hintergrund: Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 μmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

UniProt: P09571

Pathways: Transition Metal Ion Homeostasis