Luciferase Antikörper

Produktnummer ABIN5541890

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Luciferase in WB, IF, DB, EIA und ICC. Er zeigt eine Reaktivität gegenüber Photinus pyralis.

Kurzübersicht für Luciferase Antikörper (ABIN5541890)

Target: Luciferase

Reaktivität: Photinus pyralis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Luciferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Produktdetails anti-Luciferase Antikörper

Spezifität: IgG fraction of Polyclonal Rabbit antiserum to Luciferase from Photinus pyralis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Immunogen: Luciferase isolated and purified from Photinus pyralis. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Working Dilutions for Non-precipitating antibody-binding techniques: 1/1000-1/10000. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Buffer System: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Luciferase

Target: Luciferase

Hintergrund: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

UniProt: P08659