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CD11R1 Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD11R1 in FACS, IP und IHC (fro). Er zeigt eine Reaktivität gegenüber Schwein, Meerschweinchen und Human.
Produktnummer ABIN5541632

Kurzübersicht für CD11R1 Antikörper (ABIN5541632)

Target

CD11R1

Reaktivität

Schwein, Meerschweinchen, Human

Wirt

Maus

Klonalität

Monoklonal

Applikation

Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

MIL4
  • Spezifität

    This antibody recognizes the cell surface antigen classified as CD11R1 and stains Porcine eosinophils, a subset of neutrophils and NK cells. The CD11R1 antigen is a 165kD molecule, identical in size to Human CD11b. However, the expression pattern of CD11b in the Human and CD11R1 in the pig are different. Clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994). Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of 95 kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of 165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001). Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980). Species: Human, Pig, Guinea Pig.

    Aufreinigung

    Affinity Chromatography on Protein G

    Immunogen

    Porcine Lamina Propria Leucocytes

    Isotyp

    IgG1
  • Applikationshinweise

    Flow Cytometry: Use 10 μL of 1/25-1/200 diluted antibody to label 1x10 6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    PBS Preservatives: 0.09 % Sodium Azide Purity: Tissue Culture Supernatant containing 0.2M Tris/HCl pH 7.4 and 5-10 % foetal calf serum

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    Haltbarkeit

    12 months
  • Target

    CD11R1

    Andere Bezeichnung

    cd11r1
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