GOT1 Antikörper (HRP)

Produktnummer ABIN5541603

Der Schaf Polyklonal Anti-GOT1-Antikörper wurde für WB und EIA validiert. Er ist geeignet, GOT1 in Proben von Schwein zu detektieren.

Kurzübersicht für GOT1 Antikörper (HRP) (ABIN5541603)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser GOT1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-GOT1 Antikörper (HRP)

Spezifität: This antibody detects porcine GOT1. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart]. Species: Porcine (Pig).

Aufreinigung: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Aspartate aminotransferase / GOT1 from porcine heart

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot : 1/1,000-1/5,000. ELISA : 1/10,000-1/40,000. This antibody has been assayed against 1.0 μg of aspartate transaminase [porcine heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Andere Bezeichnung: got1

Hintergrund: Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.

UniProt: P00503

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process