MESP1 Antikörper (AA 1-268)

Produktnummer ABIN5541523

Dieses Anti-MESP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MESP1 in WB, ICC, IF und IHC (fro). Geeignet für Human.

Kurzübersicht für MESP1 Antikörper (AA 1-268) (ABIN5541523)

Target: MESP1 (Mesoderm Posterior 1 (MESP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MESP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MESP1 Antikörper

Bindungsspezifität: AA 1-268

Spezifität: This antibody detetcs Human MesP1. Other species not tested.

Aufreinigung: Protein A Chromatography

Immunogen: Highly pure (> 95%) recombinant Human MesP1 (Met1-Lys268) from produced in insect cells (Cat.-No AR31057PU-N).

Anwendungsinformationen

Applikationshinweise: Western blot (1-5 μg/mL). Immunofluorescence. Immunohistochemistry on Frozen Sections (1/200).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 5 mM PBS, pH 7.2 without preservatives or stabilizers

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu MESP1

Target: MESP1 (Mesoderm Posterior 1 (MESP1))

Andere Bezeichnung: mesp1

Hintergrund: ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1 (1,2). As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis (2,3). It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation (4). MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1.

Pathways: Regulation of Muscle Cell Differentiation