ESM1 Antikörper (AA 19-184)

Produktnummer ABIN5541433

Dieses Anti-ESM1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ESM1 in WB, ICC, IHC (fro) und IF. Geeignet für Human.

Kurzübersicht für ESM1 Antikörper (AA 19-184) (ABIN5541433)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ESM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Produktdetails anti-ESM1 Antikörper

Bindungsspezifität: AA 19-184

Spezifität: This antibody detects Endocan / ESM1.

Aufreinigung: Protein A chromatography

Immunogen: Highly pure (> 95%) recombinant human Endocan/ESM1 (Trp19-Arg184) derived from E. coli

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1-5 μg/mL. Immunofluorescence : 1/200. Immunhistochemistry on Frozen sections : 1:200.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 5 mM PBS, pH 7.2

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Andere Bezeichnung: esm1

Hintergrund: Endocan, also known as endothelial cell-specific molecule1 (ESM1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung. Endocan expression has also been detected in different epithelia and in adipocytes. The expression of endocan is up-regulated by TNFα, IL1β or lipopolysaccharide and down-regulated by IFNγ. The mouse Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues. The DS chain is covalently attached to serine 137. Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen1, LFA1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM1 and reducing LFA1mediated leukocyte activation. Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing Endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo. Circulating Endocan can be detected in the serum from healthy subjects.

Pathways: Growth Factor Binding