Endothelial Cells Antikörper (Biotin)

Produktnummer ABIN5541247

Der Biotin-konjugierte Ratte Monoklonal anti-Endothelial Cells Antikörper (Klon 1F10) (ABIN5541247) detektiert spezifisch Endothelial Cells in IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Endothelial Cells Antikörper (Biotin) (ABIN5541247)

Target: Endothelial Cells

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Endothelial Cells Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 1F10

Produktdetails anti-Endothelial Cells Antikörper (Biotin)

Spezifität: This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.

Immunogen: Cultured HUVECs

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen sections: 2 μg/mL (1:200). Positive control: Human placenta. Does not react on routinely processed paraffin sections.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.01 % thimerosal Stabilizers: 10 mg/mL bovine serum albumin

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Endothelial Cells

Target: Endothelial Cells

Hintergrund: The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70 % pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.