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Endothelial Cells Antikörper (Biotin)

Dieses Anti-Endothelial Cells-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Endothelial Cells in IHC (fro). Geeignet für Human.
Produktnummer ABIN5541247

Kurzübersicht für Endothelial Cells Antikörper (Biotin) (ABIN5541247)

Target

Endothelial Cells

Reaktivität

  • 4
  • 3
  • 2
Human

Wirt

  • 6
  • 3
Ratte

Klonalität

  • 9
Monoklonal

Konjugat

  • 8
  • 1
Dieser Endothelial Cells Antikörper ist konjugiert mit Biotin

Applikation

  • 9
  • 3
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

1F10
  • Spezifität

    This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.

    Immunogen

    Cultured HUVECs

    Isotyp

    IgG2a
  • Applikationshinweise

    Immunohistochemistry on Frozen sections: 2 μg/mL (1:200). Positive control: Human placenta. Does not react on routinely processed paraffin sections.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    0.01 % thimerosal Stabilizers: 10 mg/mL bovine serum albumin

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    Haltbarkeit

    12 months
  • Target

    Endothelial Cells

    Hintergrund

    The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70 % pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.
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