SNAP25 Antikörper (pSer187)

Produktnummer ABIN5541045

Der Kaninchen Polyklonal Anti-SNAP25-Antikörper wurde für WB validiert. Er ist geeignet, SNAP25 in Proben von Human, Ratte, Maus, Zebrafisch (Danio rerio), Meerschweinchen und Primate zu detektieren.

Kurzübersicht für SNAP25 Antikörper (pSer187) (ABIN5541045)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reaktivität: Human, Ratte, Maus, Zebrafisch (Danio rerio), Meerschweinchen, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SNAP25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SNAP25 Antikörper

Bindungsspezifität: pSer187

Spezifität: Specific for the ~25k SNAP25 protein phosphorylated at Ser 187 in Western blots. Immunolabeling is completely blocked by blocked λ -Ptase.

Aufreinigung: Affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser187 of SNAP25

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Andere Bezeichnung: snap25

Hintergrund: SNAP25 (Synaptosomal associated pr otein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer's Disease (Greber et al., 1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al ., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

UniProt: P60881

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport