Der Biotin-konjugierte Ziege Polyklonal anti-ALDOA Antikörper (ABIN5541022) detektiert spezifisch ALDOA in WB, IP und EIA.
Dieser Antikörper reagiert spezifisch mit Proben aus Human und Kaninchen.
This antibody detects rabbit aldolase. Cross reactivity against aldolase from other tissues and species may also occur. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [rabbit muscle]. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. Species: Rabbit, Human.
Aufreinigung
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
ALDOA
Reaktivität: Human
WB, ELISA, IHC, IF
Wirt: Kaninchen
Monoclonal
7D8
unconjugated
Recombinant Antibody
Applikationshinweise
Western blot :1/500-1/5,000. Immunoprecipitation : 1/100. Use approximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate. ELISA : 1/5,000-1/20,000. This antibody has been assayed against 1.0 μg of aldolase [rabbit muscle] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.