NEFH Antikörper

Produktnummer ABIN5540977

Dieses Anti-NEFH-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NEFH in WB und IHC (fro). Geeignet für Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Meerschweinchen, Hund, Schaf, Xenopus laevis, Hamster und Zebrafisch (Danio rerio).

Kurzübersicht für NEFH Antikörper (ABIN5540977)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Meerschweinchen, Hund, Schaf, Xenopus laevis, Hamster, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RNF405

Produktdetails anti-NEFH Antikörper

Spezifität: RNF405 reacts exclusively with the phosphorylated isoform of the 200 kD Neurofilament protein.

Aufreinigung: Purified

Immunogen: Neurofilament preparation of calf brain tissue.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin biotinylated Horseradish Peroxidase complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: neurofilament h (200 kd)

Hintergrund: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.

UniProt: P12036