Doublecortin Antikörper

Produktnummer ABIN5540930

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch Doublecortin in IF, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Doublecortin Antikörper (ABIN5540930)

Target: Doublecortin (DCX)

Reaktivität: Human, Maus

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser Doublecortin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Doublecortin Antikörper

Aufreinigung: Affinity Chromatography

Immunogen: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Immunofluorescence/ Immnunocytochemistry. Immunohistochemistry. Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at a concentration of 2 μg/mL) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions : 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Doublecortin

Target: Doublecortin (DCX)

Andere Bezeichnung: doublecortin

UniProt: O43602